| Registro completo |
| Provedor de dados: |
J. Venom. Anim. Toxins incl. Trop. Dis.
|
| País: |
Brazil
|
| Título: |
Melittin induces in vitro death of Leishmania (Leishmania) infantum by triggering the cellular innate immune response
|
| Autores: |
Pereira,Andreia Vieira
Barros,Gustavo de
Pinto,Erika Gracielle
Tempone,Andre Gustavo
Orsi,Ricardo de Oliveira
Santos,Lucilene Delazari dos
Calvi,Sueli
Ferreira Jr,Rui Seabra
Pimenta,Daniel Carvalho
Barraviera,Benedito
|
| Data: |
2016-01-01
|
| Ano: |
2016
|
| Palavras-chave: |
Melittin
Apis mellifera
Leishmania
Leishmaniasis
Peptides
Toxins
Antiparasitic
Cytokines
|
| Resumo: |
Abstract Background Apis mellifera venom, which has already been recommended as an alternative anti-inflammatory treatment, may be also considered an important source of candidate molecules for biotechnological and biomedical uses, such as the treatment of parasitic diseases. Methods Africanized honeybee venom from Apis mellifera was fractionated by RP-C18-HPLC and the obtained melittin was incubated with promastigotes and intracellular amastigotes of Leishmania (L.) infantum. Cytotoxicity to mice peritoneal macrophages was evaluated through mitochondrial oxidative activity. The production of anti- and pro-inflammatory cytokines, NO and H2O2 by macrophages was determined. Results Promastigotes and intracellular amastigotes were susceptible to melittin (IC50 28.3 μg.mL−1 and 1.4 μg.mL−1, respectively), but also showed mammalian cell cytotoxicity with an IC50 value of 5.7 μg.mL−1. Uninfected macrophages treated with melittin increased the production of IL-10, TNF-α, NO and H2O2. Infected melittin-treated macrophages increased IL-12 production, but decreased the levels of IL-10, TNF-α, NO and H2O2. Conclusions The results showed that melittin acts in vitro against promastigotes and intracellular amastigotes of Leishmania (L.) infantum. Furthermore, they can act indirectly on intracellular amastigotes through a macrophage immunomodulatory effect.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100301
|
| Editor: |
Centro de Estudos de Venenos e Animais Peçonhentos
|
| Relação: |
10.1186/s40409-016-0055-x
|
| Formato: |
text/html
|
| Fonte: |
Journal of Venomous Animals and Toxins including Tropical Diseases v.22 2016
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
